Andrew Amoscato, PhD

Research Associate Professor, Environmental and Occupational Health


130 DeSoto St, GSPH room 4104 15261
R-znvy: nnn0@cvgg.rqh
Primary Phone: 967-179-4430
Fax: 967-179-4816
Web site:

Personal Statement

Dr. Amoscato has a wealth of experience in mass
spectrometry of lipids (lipidomics), peptides, proteins (proteomics), small
molecules (metabolomics) and imaging mass spectrometry. He has applied these areas of mass spectrometric expertise to the study of lipid-protein/protein-protein
interactions, lipid alterations and phospholipid signaling during apoptosis and
autophagy. His main interests include mechanisms of apoptosis, autophagy and
ferroptosis, mitochondrial and redox biochemistry as well as the role of
oxidized lipids in these modes of cell death and. He is also interested in the role
of sphingolipids in radiation-induced apoptosis; the role of sphingolipids in
androgen ablation induced apoptosis of prostate cancer cells; the role of
lipids and lipid signaling in endothelial cell apoptosis and the role of lipid
DAMPS in inflammation and cancer.


  • 1978, Long Island University, New York, NY, B.S. Biology and Chemistry
  • 1984, University of Texas Health Science Center at Houston, Graduate School of Biomedical Sciences, Houston, TX, Ph.D. Biochemistry


Selected Publications

Thomas RL, Matsko CM, Lotze MT, Amoscato AA. Mass spectrometric
identification of increased C16 ceramide levels during apoptosis. Jounral of
Biological Chemistry. 1999; 274:30580

Matsko CM, Hunter O, Rabinoqich H, Lotze MT, Amoscato AA. Alterations in mitochondrial
lipids during Fas- and radiation-induced apoptosis. Biochemical and Biophysical
Research Communications. 2001; 287:1112-20.

Ostrander DB, Sparagna GC, Amoscato AA, McMillin JB, Dowhan W. Decreased cardiolipin synthesis
corresponds with cytochrome c release in palmitate-induced cardiomyocyte
apoptosis. The Journal of biological chemistry. 2001 Oct 12; 276 (41):38061-7.
PMID: 11500520

Kanto, T. M., P. Kalinski, O. Hunter, M. T. Lotze and A. A. Amoscato. 2001. Ceramide mediates
tumor-induced dendritic cell apoptosis. 
J. Immunol. 167: 3773-84.

Eto M, Bennouna J, Hunter OC, Hershberger PA,
Kanto T, Johnson CS, Lotze MT, Amoscato
AA. C16 ceramide accumulates following androgen ablation in LNCaP prostate
cancer cells. The Prostate. 2003 Sep 15; 57 (1):66-79. PMID: 12886525.

Lee YJ, Amoscato
AA. TRAIL and ceramide. Vitamins and hormones. 2004; 67:229-55. PMID:

  Kagan VE, Borisenko GG, Tyurina YY, Tyurin VA, Jiang J, Potapovich AI, Kini V, Amoscato AA, Fujii Y. Oxidative lipidomics of apoptosis: redox catalytic interactions of cytochrome c with
cardiolipin and phosphatidylserine. Free radical biology & medicine. 2004
Dec 15; 37 (12):1963-85. PMID: 15544916.
  Kagan VE, Tyurin VA, Jiang J, Tyurina YY, Ritov VB, Amoscato AA, Osipov AN, Belikova NA, Kapralov AA, Kini V, Vlasova II, Zhao Q, Zou M, Di P, Svistunenko DA, Kurnikov IV, Borisenko GG.
Cytochrome c acts as a cardiolipin oxygenase required for release of proapoptotic
factors. Nature chemical biology. 2005 Sep; 1 (4):223-32. PMID: 16408039.

Bayir H, Tyurin VA, Tyurina YY, Viner R, Ritov, V, Amoscato AA, Zhao Q, Zhang XJ,
Janesko-Feldman KL, Alexander H, Basova LV, Clark RS, Kochanek PM, Kagan VE. Selective
early cardiolipin peroxidation after traumatic brain injury: an oxidative
lipidomics analysis. Annals of neurology. 2007 Aug; 62 (2):154-69. PMID:

Bayir H, Kapralov AA, Jiang J, Huang Z, Tyurina YY, Tyurin VA, Zhao Q, Belikova NA, Vlasova II, Maeda A, Zhu J, Na HM, Mastroberardino PG, Sparvero LJ, Amoscato
AA, Chu CT, Greenamyre JT, Kagan VE. Peroxidase mechanism of
lipid-dependent cross-linking of synuclein with cytochrome C: protection against
apoptosis versus delayed oxidative stress in Parkinson disease. The Journal of
biological chemistry. 2009 Jun 5; 284 (23):15951-69. PMCID: PMC2708890. PMID:

Sparvero LJ, Amoscato AA, Kochanek PM, Pitt BR, Kagan VE, Bayir H.
Mass-spectrometry based oxidative lipidomics and lipid imaging: applications in
traumatic brain injury. Journal of neurochemistry. 2010 Dec; 115 (6):1322-36.
PMCID: PMC3285274. PMID: 20950335.


Atkinson J, Kapralov AA, Yanamala N, Tyurina YY, Amoscato AA, Pearce L, Peterson
J, Huang Z, Jiang J, Samhan-Arias AK, Maeda A, Feng W, Wasserloos K, Belikova
NA, Tyurin VA, Wang H, Fletcher J, Wang Y, Vlasova II, Klein-Seetharaman J,
Stoyanovsky DA, Bayîr H, Pitt BR, Epperly MW,7Greenberger JS, Kagan VE. A
mitochondria-targeted inhibitor of cytochrome c perxidase mitigates
radiation-induced death. Nature communications. 2011; 2:497. PMID: 21988913.

Samhan-Arias AK, Ji J, Demidova OM, Sparvero LJ, Feng W, Tyurin V, Tyurina YY, Epperly MW, Shvedova AA, Greenberger JS, Bayir H, Kagan VE, Amoscato AA.
Oxidized phospholipids as biomarkers of tissue and cell damage with a focus on
cardiolipin. Biochimica et biophysica acta. 2012 Mar 23. PMID: 22464971.

  Sparvero LJ, Amoscato AA, Dixon CE, Long
JB, Kochanek PM, Pitt BR, Bayir H, Kagan VE. Mapping of phospholipids by
MALDI imaging (MALDI-MSI): realities and expectations. Chemistry and physics
of lipids. 2012 Jul; 165 (5):545-62. PMCID: PMC3642772. PMID: 22692104.
 Ji J, Kline AE, Amoscato A, Samhan-Arias
AK, Sparvero LJ, Tyurin VA, Tyurina YY, Fink B, Manole MD, Puccio AM, Okonkwo
DO, Cheng JP, Alexander H, Clark RS, Kochanek PM, Wipf P, Kagan VE, Bayir H.
Lipidomics identifies cardiolipin oxidation as a mitochondrial target for
redox therapy of brain injury. Nature neuroscience. 2012 Oct; 15
(10):1407-13. PMID: 22922784.

Schlattner U, Tokarska-Schlattner M, Ramirez S, Tyurina YY, Amoscato AA,
Mohammadyani D, Huang Z, Jiang J, Yanamala N, Seffouh A, Boissan M, Epand RF,
Epand RM, Klein-Seetharaman J, Lacombe ML, Kagan VE. Dual function of
mitochondrial Nm23-H4 protein in phosphotransfer and intermembrane lipid
transfer: a cardiolipin-dependent switch. The Journal of biological chemistry.
2013 Jan 4; 288 (1):111-21. PMCID: PMC3537004. PMID: 23150663.

Chu, CT, Ji, J, Dagda, RK, Jiang, JF, Amoscato, AA, Kapralov, OK, Yanamala,
N, Klein-Seetharaman, J, Balasubramaniam, K, Borisenko, G, Huang, Z, Baty, C,
Watkins, S, Bayir, H and Kagan, VE Cardiolipin externalization as an
elimination signal for mammalian mitophagy. Nat. Cell Biol. 2013 15:1197-1205

Amoscato, AA, Sparvero, LJ, He, RR, Watkins, SW, Bayir, H and Kagan, VE. Imaging mass spectrometry of diversified cardiolipin molecular species in the brain. Analytical Chemistry 2014.

Stoyanovsky, DA, Sparvero, LJ, Amoscato, AA,
He, RR, Watkins, S, Pitt, BR, Bayir, H, and Kagan, VE Improved spatial
resolution of MALDI imaging of lipids in the brain by alkylated derivatives
of 2,5-dihydroxybenszoic acid. Rapid Comm. Mass Spectrom. 2014. 28:403-41

Cao, W, Ramakrishnan, R, Tuyrin, VA, Veglia, F, Condamine, T, Amoscato, A, Mohammadyani, D,
Johnson, JJ, Zhang, LM,  Judith Klein-Seetharaman, J, Celis, E,
Valerian E. Kagan, VE  and Gabrilovich, DI. Oxidized lipids block antigen cross-presentation by dendritic cells in cancer. J. Immunol. 2014

Jiang, J, Bakan , A, Kapralov, AA, Ishara, KI, Huang, Z, Amoscato, AA,  Peterson, J, Garapati, VK, Saxena, SK, Bayir, H, Atkinson,  J, Bahar, I,
Kagan, VE. Designing Inhibitors of Cytochrome c/Cardiolipin Peroxidase
Complexes:  Mitochondria-Targeted Imidazole-Substituted Fatty Acids.
Free Rad Biol Med. 2014. 71:221-230.

Tyurina, YY, Domingues, RM, Tyurin, VA, Maciel, E, Domingues, P, Amoscato, AA, Bayir, H, and Kagan, VE Characterization of cardiolipins and their oxidation products by LC-MS analysis. Chem. Phys. Lipids. 2014. 179:3-10.

Amoscato, AA,Sparvero, LJ, He, RR, Watkins, SW, Bayir, H and Kagan, VE. Imaging mass
spectrometry of diversified cardiolipin molecular species in the brain.
Analytical Chemistry 2014. 86:6587-95.

Mohammadyani, D., Tyurin, V. A., Amoscato, A. A.,
Gabrilovich, D. I., Klein-Seetharaman, J. and Kagan, V. E. Computational
Approaches to Understanding the Role of Oxidized Tri-Aclyglycerols in Suppression of Antigen Cross-Presentation in Cancer Biophysical
Journal 2015. 106:805a-806a.

Rolyan, H., Tyurina, Y., Hernandez, M., Amoscato,
A., Sparvero, L., Nmezi, B., Lu, Y., Estécio, M., Lin, K., Chen, J., He,
R., Pin Gong, Rigatti, L., Dupree J., Bayir, H., Kagan, V., Casaccia, P., and Padiath, Q., Defects of lipid synthesis underlie the age dependent
demyelination caused by lamin B1 Over expression. 2015. J. Neuroscience 35:12002-12017.

Mao,G., Qu, F., St. Croix, C., Tyurina, Y., Jiang, J., Huang, Z., Amoscato, A. A., Cheikhi, A., Bayir, H. and Kagan, V. Mitochondrial redox opto-lipidomics reveals selectively mono-oxygenated cardiolipins as pro-apoptotic death signals. ACS Chem. Biol. 2016. 11:530-40.

Buland,J., Wasserloos, K, Tyurin, V., Tyurina, Y., Amoscato, A. A., Ofori-Acquah, S. Kagan, V., and Pitt, B.Biosynthesis of Oxidized Lipid Mediators via Lipoprotein Associated Phospholipase A2 Hydrolysis of Extracellular Cardiolipin Induces Endothelial Toxicity. Am J Physiol
Lung Cell Mol Physiol 2016 Aug 27;311(2):L303-16.

L. J. Sparvero, A. A. Amoscato, A. B. Fink, T. Anthonymuthu, S. Watkins,
V.E. Kagan, and  H. Bayir. Imaging Mass Spectrometry Reveals a Massive Loss of Polyunsaturated Cardiolipins in the Immediate Cortical Contusion Area and in the Distal Hippocampal and Thalamic
Regions after Traumatic Brain Injury J. Neurochemistry 139:659-75, 2016

Valerian E. Kagan, Gaowei Mao, Feng Qu, Jose Pedro Friedmann Angeli, Sebastian Doll Claudette
St Croix, Haider Dar, Bing Liu, Vladimir A. Tyurin, Vladimir B. Ritov,Olexandr A. Kapralov, Andrew A.
Amoscato, Jianfei Jiang, Tamil Anthonymuthu, Dariush Mohammadyani, Qin Yang, Judith Klein-Seetharaman, Simon Watkins, Ivet Bahar, Joel Greenberger, Rama Mallampalli, Brent R. Stockwell, Yulia Y. Tyurina, Marcus Conrad, Hülya Bayır. Lipid Death Signals in Ferroptosis Unearthed: Oxygenated Arachidonoyl- and Adrenoyl-Diacyl-Phosphatidylethanolamines. Nat. Chem. Biol. 13, 81-90, 2017

John Maguire, Yulia Tyurina, Dariush Mohammadyani, Aleksandr A Kapralov, Tamil S Anthonymuthu, Feng Qu, Andrew A Amoscato, Louis J Sparvero, Vladimir A Tyurin, Joan Planas-Iglesias, Rong-Rong He, Judith Klein-Seetharaman, Hülya Bayır, Valerian E. Kagan. Known Unknowns of Cardiolipin Signaling: The Best Is Yet To Come. Biochim. Biophys. Acta, 1862:8-24 2016

Hua Tian, Louis J. Sparvero, Andrew A. Amoscato, Anna Bloom, Hülya Bayır, Valerian E. Kagan, and Nicholas Winograd. Gas Cluster Ion Beam Time-of-Flight Secondary Ion Mass Spectrometry High-Resolution Imaging of Cardiolipin Speciation in the Brain: Identification of Molecular Losses
after Traumatic Injury Anal. Chem., 2017, 89(8), pp 4611–4619

Sally E. Wenzel, Yulia Y. Tyurina, Jinming Zhao, Claudette M. St. Croix, Haider H. Dar, Gaowei Mao, Vladimir A. Tyurin, Tamil S. Anthonymuthu, Alexandr A. Kapralov, Andrew A. Amoscato, Karolina Mikulska-Ruminska, Indira H. Shrivastava, Elizabeth M. Kenny, Qin Yang, Joel C. Rosenbaum, Louis J. Sparvero, David R. Emlet, Xiaoyan Wen, Yoshinori Minami, Feng Qu, Simon C. Watkins, Theodore R. Holman, Andrew P. VanDemark, John A. Kellum, Ivet Bahar, Hu¨ lya Bayır, and Valerian E. Kagan. PEBP wardens ferroptosis by enabling lipoxygenase
generation o lipid death signals. Cell 2017, 171, 628-641.

Laura K Cole; Jin Hee Kim; Andrew A Amoscato; Yulia Y Tyurina; Hulya Bayir; Benyamin Karimi; Tabrez Siddiqui; Valerian E Kagan; Grant Michael Hatch; Tiina M Kauppinen. Aberrant cardiolipin metabolism is associated with cognitive deficiency and hippocampal alteration in tafazzin knockdown mice. BBA-Molecular Basis of Disease. Biophys. Acta: Molecular Basis of Disease. 1864:3353-67, 2018.

Anthonymuthu, Tamil; Kenny, Elizabeth; Shrivastava, Indira; Tyurina, Yulia; Hier, Zachary; Ting, Hsiu-Chi; Tyurin, Vladimir; Nesterova, Anastasia; Amoscato, Andrew; Mikulska-Ruminska, Karolina; Rosenbaum, Joel; Conrad, Marcus; Kellum, John; Wenzel,  Sally ; VanDemark , Andrew; Bahar, Ivet; Kagan, Valerian; Bayir, Hulya. Empowerment of 15-lipoxygenase catalytic competence in selective oxidation of membrane ETE-PE to ferroptotic death signals, HpETE-PE. J. Am. Chem Soc. 2018, 140 (51), 17835-17839 DOI: 10.1021/jacs.8b09913

Tamil S. Anthonymuthu, Elizabeth M. Kenny, Andrew M. Lamade, Hitesh Gidwani, Nicholas M. Krehel, Amalea Misse, Xiaotian Gao, Andrew A. Amoscato, Adam C. Straub, Valerian E. Kagan, Cameron Dezfulian, Hülya Bayır. Lipidomics Detection of Brain Cardiolipins in Plasma Is Associated with Outcome after Cardiac Arrest. Critical Care Medicine 2019, 47, e292-e300.

Honglu Chao, Tamil S. Anthonymuthu, Elizabeth M. Kenny, Andrew A. Amoscato,Laura K. Cole, Grant M. Hatch, Jing Ji, Valerian E. Kagan and Hülya Bayır. Disentangling oxidation/hydrolysis reactions of brain mitochondrial cardiolipins in pathogenesis of traumatic injury. J. Clin. Investigation. Insight. 2018. 3(21), 1-15.
Tian Hua, L.J. Sparvero, Paul Blenkinsopp, Andew A. Amoscato, Simon C. Watkins, Hulya Bayir, valerian E. Kagan and Nicholas Winograd. Secondary ion mass spectrometry images cardiolipins and phosphatidylethanolamines at the subcellular level. Angewandte Chemie 2019, 131(10), 3188-3193.

Yulia Y. Tyurina,  Claudette St.M. Croix,  Simon C.Watkins,  Alan M.Watson,

MichaelW. Epperly,  Tamil S. Anthonymuthu,  Elena R. Kisin,  Irina I. Vlasova,

Olga Krysko,  Dmitri V. Krysko,  Alexandr A. Kapralov,  Haider H. Dar,

Vladimir A. Tyurin,  Andrew A. Amoscato,  Elena N. Popova,

Sergey B. Bolevich,  Peter S. Timashev,  John A. Kellum,  Sally E.Wenzel,

Ramapalla K. Mallampalli,  Joel S. Greenberger,  Hulya Bayir,

Anna A. Shvedova,  Valerian E. Kagan. Redox (phospho)lipidomics of signaling in inflammation and programmed cell death. 2019, J. Leuckocyte Biol. 106: 57-81. doi: 10.1002/JLB.3MIR0119-004RR


Anthonymuthu TS, Kenny EM, Amoscato AA, Lewis J, Kochanek PM, Kagan VE, Bayır H. Global assessment of oxidized free fatty acids in brain reveals an enzymatic predominance to oxidative signaling after trauma. Biochim Biophys Acta Mol Basis Dis. 2017 Oct;1863(10 Pt B):2601-2613. doi: 10.1016/j.bbadis.2017.03.015. Epub 2017 Mar 25.



Anthonymuthu TS, Kenny EM, Lamade AM, Gidwani H, Krehel NM, Misse A, Gao X, Amoscato AA, Straub AC, Kagan VE, Dezfulian C, Bayır H. Lipidomics Detection of Brain Cardiolipins in Plasma Is Associated With Outcome After Cardiac Arrest. Crit Care Med. 2019 Apr;47(4):e292-e300. doi: 10.1097/CCM.0000000000003636.


Tyurina YY, Tyurin VA, Anthonymuthu T, Amoscato AA, Sparvero LJ, Nesterova AM, Baynard ML, Sun W, He R, Khaitovich P, Vladimirov YA, Gabrilovich DI, Bayır H, Kagan VE. "Redox lipidomics technology: Looking for a needle in a haystack". Chem Phys Lipids. 2019 Jul;221:93-107. doi: 10.1016/j.chemphyslip.2019.03.012. Epub 2019 Mar 27. 



Tyurina YY, St Croix CM, Watkins SC, Watson AM, Epperly MW, Anthonymuthu TS, Kisin ER, Vlasova II, Krysko O, Krysko DV, Kapralov AA, Dar HH, Tyurin VA, Amoscato AA, Popova EN, Bolevich SB, Timashev PS, Kellum JA, Wenzel SE, Mallampalli RK, Greenberger JS, Bayir H, Shvedova AA, Kagan VE. Redox (phospho)lipidomics of signaling in inflammation and programmed cell death.

J Leukoc Biol. 2019 Jul;106(1):57-81. doi: 10.1002/JLB.3MIR0119-004RR. Epub 2019 May 9. 



Kagan VE, Tyurina YY, Sun WY, Vlasova II, Dar H, Tyurin VA, Amoscato AA, Mallampalli R, van der Wel PCA, He RR, Shvedova AA, Gabrilovich DI, Bayir H. Redox phospholipidomics of enzymatically generated oxygenated phospholipids as specific signals of programmed cell death. Free Radic Biol Med. 2020 Feb 1;147:231-241. doi: 10.1016/j.freeradbiomed.2019.12.028. Epub 2019 Dec 25. 


Bayır H, Anthonymuthu TS, Tyurina YY, Patel SJ, Amoscato AA, Lamade AM, Yang Q, Vladimirov GK, Philpott CC, Kagan VE. Achieving Life through Death: Redox Biology of Lipid Peroxidation in Ferroptosis. Cell Chem Biol. 2020 Apr 16;27(4):387-408. doi: 10.1016/j.chembiol.2020.03.014. Epub 2020 Apr 9. 





Andrew  Amoscato