Andrew Amoscato, PhD

Research Associate Professor, Environmental and Occupational Health


Public Health Building 4104, 130 De Soto Street, Pittsburgh, PA 15261
R-znvy: nnn0@cvgg.rqh
Primary Phone: 967-179-4430
Fax: 967-179-4816
Web site:

Personal Statement

Dr. Amoscato has a wealth of experience in mass spectrometry of lipids (lipidomics), peptides, proteins (proteomics), small molecules (metabolomics) and imaging mass spectrometry. He has applied these areas of mass spectrometric expertise to the study of lipid-protein/protein-protein interactions, lipid alterations and phospholipid signaling during apoptosis and autophagy. His main interests include mechanisms of apoptosis, autophagy and ferroptosis, mitochondrial and redox biochemistry as well as the role of oxidized lipids in these modes of cell death and. He is also interested in the role of sphingolipids in radiation-induced apoptosis; the role of sphingolipids in androgen ablation induced apoptosis of prostate cancer cells; the role of lipids and lipid signaling in endothelial cell apoptosis and the role of lipid DAMPS in inflammation and cancer.


  • 1978 | Long Island University, New York, NY | B.S. Biology and Chemistry
  • 1984 | University of Texas Health Science Center at Houston, Graduate School of Biomedical Sciences, Houston, TX | Ph.D. Biochemistry


Selected Publications

Thomas RL, Matsko CM, Lotze MT, Amoscato AA. Mass spectrometric identification of increased C16 ceramide levels during apoptosis. Jounral of Biological Chemistry. 1999; 274:30580


Matsko CM, Hunter O, Rabinoqich H, Lotze MT, Amoscato AA. Alterations in mitochondrial lipids during Fas- and radiation-induced apoptosis. Biochemical and Biophysical Research Communications. 2001; 287:1112-20.


Ostrander DB, Sparagna GC, Amoscato AA, McMillin JB, Dowhan W. Decreased cardiolipin synthesis corresponds with cytochrome c release in palmitate-induced cardiomyocyte apoptosis. The Journal of biological chemistry. 2001 Oct 12; 276 (41):38061-7. PMID: 11500520


Kanto, T. M., P. Kalinski, O. Hunter, M. T. Lotze and Amoscato AA. 2001. Ceramide mediates tumor-induced dendritic cell apoptosis. J. Immunol. 167: 3773-84.


Eto M, Bennouna J, Hunter OC, Hershberger PA, Kanto T, Johnson CS, Lotze MT, Amoscato AA. C16 ceramide accumulates following androgen ablation in LNCaP prostate cancer cells. The Prostate. 2003 Sep 15; 57 (1):66-79. PMID: 12886525.


Lee YJ, Amoscato AA. TRAIL and ceramide. Vitamins and hormones. 2004; 67:229-55. PMID: 15110180.


Kagan VE, Borisenko GG, Tyurina YY, Tyurin VA, Jiang J, Potapovich AI, Kini V, Amoscato AA, Fujii Y. Oxidative lipidomics of apoptosis: redox catalytic interactions of cytochrome c with cardiolipin and phosphatidylserine. Free radical biology & medicine. 2004 Dec 15; 37 (12):1963-85. PMID: 15544916.


Kagan VE, Tyurin VA, Jiang J, Tyurina YY, Ritov VB, Amoscato AA, Osipov AN, Belikova NA, Kapralov AA, Kini V, Vlasova II, Zhao Q, Zou M, Di P, Svistunenko DA, Kurnikov IV, Borisenko GG. Cytochrome c acts as a cardiolipin oxygenase required for release of proapoptotic factors. Nature chemical biology. 2005 Sep; 1 (4):223-32. PMID: 16408039.


Bayir H, Tyurin VA, Tyurina YY, Viner R, Ritov, V, Amoscato AA, Zhao Q, Zhang XJ, Janesko-Feldman KL, Alexander H, Basova LV, Clark RS, Kochanek PM, Kagan VE. Selective early cardiolipin peroxidation after traumatic brain injury: an oxidative lipidomics analysis. Annals of neurology. 2007 Aug; 62 (2):154-69. PMID: 17685468.


Bayir H, Kapralov AA, Jiang J, Huang Z, Tyurina YY, Tyurin VA, Zhao Q, Belikova NA, Vlasova II, Maeda A, Zhu J, Na HM, Mastroberardino PG, Sparvero LJ, Amoscato AA, Chu CT, Greenamyre JT, Kagan VE. Peroxidase mechanism of lipid-dependent cross-linking of synuclein with cytochrome C: protection against apoptosis versus delayed oxidative stress in Parkinson disease. The Journal of biological chemistry. 2009 Jun 5; 284 (23):15951-69. PMCID: PMC2708890. PMID: 19351880.


Sparvero LJ, Amoscato AA, Kochanek PM, Pitt BR, Kagan VE, Bayir H. Mass-spectrometry based oxidative lipidomics and lipid imaging: applications in traumatic brain injury. Journal of neurochemistry. 2010 Dec; 115 (6):1322-36. PMCID: PMC3285274. PMID: 20950335.


Atkinson J, Kapralov AA, Yanamala N, Tyurina YY, Amoscato AA, Pearce L, Peterson J, Huang Z, Jiang J, Samhan-Arias AK, Maeda A, Feng W, Wasserloos K, Belikova NA, Tyurin VA, Wang H, Fletcher J, Wang Y, Vlasova II, Klein-Seetharaman J, Stoyanovsky DA, Bayîr H, Pitt BR, Epperly MW,7Greenberger JS, Kagan VE. A mitochondria-targeted inhibitor of cytochrome c perxidase mitigates radiation-induced death. Nature communications. 2011; 2:497. PMID: 21988913.


Samhan-Arias AK, Ji J, Demidova OM, Sparvero LJ, Feng W, Tyurin V, Tyurina YY, Epperly MW, Shvedova AA, Greenberger JS, Bayir H, Kagan VE, Amoscato AA. Oxidized phospholipids as biomarkers of tissue and cell damage with a focus on cardiolipin. Biochimica et biophysica acta. 2012 Mar 23. PMID: 22464971.


Sparvero LJ, Amoscato AA, Dixon CE, Long JB, Kochanek PM, Pitt BR, Bayir H, Kagan VE. Mapping of phospholipids by MALDI imaging (MALDI-MSI): realities and expectations. Chemistry and physics of lipids. 2012 Jul; 165 (5):545-62. PMCID: PMC3642772. PMID: 22692104.


Ji J, Kline AE, Amoscato AA, Samhan-Arias AK, Sparvero LJ, Tyurin VA, Tyurina YY, Fink B, Manole MD, Puccio AM, Okonkwo DO, Cheng JP, Alexander H, Clark RS, Kochanek PM, Wipf P, Kagan VE, Bayir H. Lipidomics identifies cardiolipin oxidation as a mitochondrial target for redox therapy of brain injury. Nature neuroscience. 2012 Oct; 15 (10):1407-13. PMID: 22922784.


Schlattner U, Tokarska-Schlattner M, Ramirez S, Tyurina YY, Amoscato AA, Mohammadyani D, Huang Z, Jiang J, Yanamala N, Seffouh A, Boissan M, Epand RF, Epand RM, Klein-Seetharaman J, Lacombe ML, Kagan VE. Dual function of mitochondrial Nm23-H4 protein in phosphotransfer and intermembrane lipid transfer: a cardiolipin-dependent switch. The Journal of biological chemistry. 2013 Jan 4; 288 (1):111-21. PMCID: PMC3537004. PMID: 23150663.


Chu, CT, Ji, J, Dagda, RK, Jiang, JF, Amoscato AA, Kapralov, OK, Yanamala, N, Klein-Seetharaman, J, Balasubramaniam, K, Borisenko, G, Huang, Z, Baty, C, Watkins, S, Bayir, H and Kagan, VE Cardiolipin externalization as an elimination signal for mammalian mitophagy. Nat. Cell Biol. 2013 15:1197-1205


Amoscato AA, Sparvero, LJ, He, RR, Watkins, SW, Bayir, H and Kagan, VE. Imaging mass spectrometry of diversified cardiolipin molecular species in the brain. Analytical Chemistry 2014. 86:6587-95.


Stoyanovsky, DA, Sparvero, LJ, Amoscato AA, He, RR, Watkins, S, Pitt, BR, Bayir, H, and Kagan, VE Improved spatial resolution of MALDI imaging of lipids in the brain by alkylated derivatives of 2,5-dihydroxybenszoic acid. Rapid Comm. Mass Spectrom. 2014. 28:403-41


Cao, W, Ramakrishnan, R, Tuyrin, VA, Veglia, F, Condamine, T, Amoscato AA, Mohammadyani, D, Johnson, JJ, Zhang, LM, Judith Klein-Seetharaman, J, Celis, E, Valerian E. Kagan, VE and Gabrilovich, DI. Oxidized lipids block antigen cross-presentation by dendritic cells in cancer. J. Immunol. 2014 192:2920-31.


Jiang, J, Bakan , A, Kapralov, AA, Ishara, KI, Huang, Z, Amoscato AA, Peterson, J, Garapati, VK, Saxena, SK, Bayir, H, Atkinson, J, Bahar, I, Kagan, VE. Designing Inhibitors of Cytochrome c/Cardiolipin Peroxidase Complexes:  Mitochondria-Targeted Imidazole-Substituted Fatty Acids. Free Rad Biol Med. 2014. 71:221-230.


Tyurina, YY, Domingues, RM, Tyurin, VA, Maciel, E, Domingues, P, Amoscato AA, Bayir, H, and Kagan, VE Characterization of cardiolipins and their oxidation products by LC-MS analysis. Chem. Phys. Lipids. 2014. 179:3-10.


Amoscato AA, Sparvero, LJ, He, RR, Watkins, SW, Bayir, H and Kagan, VE. Imaging mass
spectrometry of diversified cardiolipin molecular species in the brain.
Analytical Chemistry 2014. 86:6587-95.


Mohammadyani, D., Tyurin, V. A., Amoscato AA, Gabrilovich, D. I., Klein-Seetharaman, J. and Kagan, V. E. Computational Approaches to Understanding the Role of Oxidized Tri-Aclyglycerols in Suppression of Antigen Cross-Presentation in Cancer Biophysical Journal 2015. 106:805a-806a.


Rolyan, H., Tyurina, Y., Hernandez, M., Amoscato AA, Sparvero, L., Nmezi, B., Lu, Y., Estécio, M., Lin, K., Chen, J., He, R., Pin Gong, Rigatti, L., Dupree J., Bayir, H., Kagan, V., Casaccia, P., and Padiath, Q., Defects of lipid synthesis underlie the age dependent demyelination caused by lamin B1 Over expression. 2015. J. Neuroscience 35:12002-12017.


Mao,G., Qu, F., St. Croix, C., Tyurina, Y., Jiang, J., Huang, Z., Amoscato AA, Cheikhi, A., Bayir, H. and Kagan, V. Mitochondrial redox opto-lipidomics reveals selectively mono-oxygenated cardiolipins as pro-apoptotic death signals. ACS Chem. Biol. 2016. 11:530-40.


Buland,J., Wasserloos, K, Tyurin, V., Tyurina, Y., Amoscato AA, Ofori-Acquah, S. Kagan, V., and Pitt, B.Biosynthesis of Oxidized Lipid Mediators via Lipoprotein Associated Phospholipase A2 Hydrolysis of Extracellular Cardiolipin Induces Endothelial Toxicity. Am J Physiol Lung Cell Mol Physiol 2016 Aug 27;311(2):L303-16.


L. J. Sparvero, Amoscato AA, A. B. Fink, T. Anthonymuthu, S. Watkins, V.E. Kagan, and H. Bayir. Imaging Mass Spectrometry Reveals a Massive Loss of Polyunsaturated Cardiolipins in the Immediate Cortical Contusion Area and in the Distal Hippocampal and Thalamic Regions after Traumatic Brain Injury J. Neurochemistry 139:659-75, 2016


Valerian E. Kagan, Gaowei Mao, Feng Qu, Jose Pedro Friedmann Angeli, Sebastian Doll Claudette St Croix, Haider Dar, Bing Liu, Vladimir A. Tyurin, Vladimir B. Ritov,Olexandr A. Kapralov, Amoscato AA, Jianfei Jiang, Tamil Anthonymuthu, Dariush Mohammadyani, Qin Yang, Judith Klein-Seetharaman, Simon Watkins, Ivet Bahar, Joel Greenberger, Rama Mallampalli, Brent R. Stockwell, Yulia Y. Tyurina, Marcus Conrad, Hülya Bayır. Lipid Death Signals in Ferroptosis Unearthed: Oxygenated Arachidonoyl- and Adrenoyl-Diacyl-Phosphatidylethanolamines. Nat. Chem. Biol. 13, 81-90, 2017


John Maguire, Yulia Tyurina, Dariush Mohammadyani, Aleksandr A Kapralov, Tamil S Anthonymuthu, Feng Qu, Amoscato AA, Louis J Sparvero, Vladimir A Tyurin, Joan Planas-Iglesias, Rong-Rong He, Judith Klein-Seetharaman, Hülya Bayır, Valerian E. Kagan. Known Unknowns of Cardiolipin Signaling: The Best Is Yet To Come. Biochim. Biophys. Acta, 1862:8-24 2016


Hua Tian, Louis J. Sparvero, Amoscato AA, Anna Bloom, Hülya Bayır, Valerian E. Kagan, and Nicholas Winograd. Gas Cluster Ion Beam Time-of-Flight Secondary Ion Mass Spectrometry High-Resolution Imaging of Cardiolipin Speciation in the Brain: Identification of Molecular Losses after Traumatic Injury Anal. Chem., 2017, 89(8), pp 4611–4619


Sally E. Wenzel, Yulia Y. Tyurina, Jinming Zhao, Claudette M. St. Croix, Haider H. Dar, Gaowei Mao, Vladimir A. Tyurin, Tamil S. Anthonymuthu, Alexandr A. Kapralov, Amoscato AA, Karolina Mikulska-Ruminska, Indira H. Shrivastava, Elizabeth M. Kenny, Qin Yang, Joel C. Rosenbaum, Louis J. Sparvero, David R. Emlet, Xiaoyan Wen, Yoshinori Minami, Feng Qu, Simon C. Watkins, Theodore R. Holman, Andrew P. VanDemark, John A. Kellum, Ivet Bahar, Hu¨ lya Bayır, and Valerian E. Kagan. PEBP wardens ferroptosis by enabling lipoxygenase generation o lipid death signals. Cell 2017, 171, 628-641.


Laura K Cole; Jin Hee Kim; Amoscato AA; Yulia Y Tyurina; Hulya Bayir; Benyamin Karimi; Tabrez Siddiqui; Valerian E Kagan; Grant Michael Hatch; Tiina M Kauppinen. Aberrant cardiolipin metabolism is associated with cognitive deficiency and hippocampal alteration in tafazzin knockdown mice. BBA-Molecular Basis of Disease. Biophys. Acta: Molecular Basis of Disease. 1864:3353-67, 2018.


Anthonymuthu, Tamil; Kenny, Elizabeth; Shrivastava, Indira; Tyurina, Yulia; Hier, Zachary; Ting, Hsiu-Chi; Tyurin, Vladimir; Nesterova, Anastasia; Amoscato AA; Mikulska-Ruminska, Karolina; Rosenbaum, Joel; Conrad, Marcus; Kellum, John; Wenzel, Sally ; VanDemark , Andrew; Bahar, Ivet; Kagan, Valerian; Bayir, Hulya. Empowerment of 15-lipoxygenase catalytic competence in selective oxidation of membrane ETE-PE to ferroptotic death signals, HpETE-PE. J. Am. Chem Soc. 2018, 140 (51), 17835-17839 DOI: 10.1021/jacs.8b09913


Tamil S. Anthonymuthu, Elizabeth M. Kenny, Andrew M. Lamade, Hitesh Gidwani, Nicholas M. Krehel, Amalea Misse, Xiaotian Gao, Amoscato AA, Adam C. Straub, Valerian E. Kagan, Cameron Dezfulian, Hülya Bayır. Lipidomics Detection of Brain Cardiolipins in Plasma Is Associated with Outcome after Cardiac Arrest. Critical Care Medicine 2019, 47, e292-e300.


Honglu Chao, Tamil S. Anthonymuthu, Elizabeth M. Kenny, Amoscato AA, Laura K. Cole, Grant M. Hatch, Jing Ji, Valerian E. Kagan and Hülya Bayır. Disentangling oxidation/hydrolysis reactions of brain mitochondrial cardiolipins in pathogenesis of traumatic injury. J. Clin. Investigation. Insight. 2018. 3(21), 1-15.


Tian Hua, L.J. Sparvero, Paul Blenkinsopp, Amoscato AA, Simon C. Watkins, Hulya Bayir, valerian E. Kagan and Nicholas Winograd. Secondary ion mass spectrometry images cardiolipins and phosphatidylethanolamines at the subcellular level. Angewandte Chemie 2019, 131(10), 3188-3193.


Yulia Y. Tyurina, Claudette St.M. Croix, Simon C.Watkins, Alan M.Watson, Michael W. Epperly, Tamil S. Anthonymuthu, Elena R. Kisin, Irina I. Vlasova, Olga Krysko, Dmitri V. Krysko, Alexandr A. Kapralov, Haider H. Dar, Vladimir A. Tyurin, Amoscato AA, Elena N. Popova, Sergey B. Bolevich, Peter S. Timashev, John A. Kellum, Sally E.Wenzel, Ramapalla K. Mallampalli, Joel S. Greenberger, Hulya Bayir, Anna A. Shvedova, Valerian E. Kagan. Redox (phospho)lipidomics of signaling in inflammation and programmed cell death. 2019, J. Leuckocyte Biol. 106: 57-81. doi: 10.1002/JLB.3MIR0119-004RR


Anthonymuthu TS, Kenny EM, Amoscato AA, Lewis J, Kochanek PM, Kagan VE, Bayır H. Global assessment of oxidized free fatty acids in brain reveals an enzymatic predominance to oxidative signaling after trauma. Biochim Biophys Acta Mol Basis Dis. 2017 Oct;1863(10 Pt B):2601-2613. doi: 10.1016/j.bbadis.2017.03.015. Epub 2017 Mar 25.


Anthonymuthu TS, Kenny EM, Lamade AM, Gidwani H, Krehel NM, Misse A, Gao X, Amoscato AA, Straub AC, Kagan VE, Dezfulian C, Bayır H. Lipidomics Detection of Brain Cardiolipins in Plasma Is Associated With Outcome After Cardiac Arrest. Crit Care Med. 2019 Apr;47(4):e292-e300. doi: 10.1097/CCM.0000000000003636.


Tyurina YY, Tyurin VA, Anthonymuthu T, Amoscato AA, Sparvero LJ, Nesterova AM, Baynard ML, Sun W, He R, Khaitovich P, Vladimirov YA, Gabrilovich DI, Bayır H, Kagan VE. "Redox lipidomics technology: Looking for a needle in a haystack". Chem Phys Lipids. 2019 Jul;221:93-107. doi: 10.1016/j.chemphyslip.2019.03.012. Epub 2019 Mar 27.


Tyurina YY, St Croix CM, Watkins SC, Watson AM, Epperly MW, Anthonymuthu TS, Kisin ER, Vlasova II, Krysko O, Krysko DV, Kapralov AA, Dar HH, Tyurin VA, Amoscato AA, Popova EN, Bolevich SB, Timashev PS, Kellum JA, Wenzel SE, Mallampalli RK, Greenberger JS, Bayir H, Shvedova AA, Kagan VE. Redox (phospho)lipidomics of signaling in inflammation and programmed cell death. J Leukoc Biol. 2019 Jul;106(1):57-81. doi: 10.1002/JLB.3MIR0119-004RR. Epub 2019 May 9. 


Kagan VE, Tyurina YY, Sun WY, Vlasova II, Dar H, Tyurin VA, Amoscato AA, Mallampalli R, van der Wel PCA, He RR, Shvedova AA, Gabrilovich DI, Bayir H. Redox phospholipidomics of enzymatically generated oxygenated phospholipids as specific signals of programmed cell death. Free Radic Biol Med. 2020 Feb 1;147:231-241. doi: 10.1016/j.freeradbiomed.2019.12.028. Epub 2019 Dec 25.


Bayır H, Anthonymuthu TS, Tyurina YY, Patel SJ, Amoscato AA, Lamade AM, Yang Q, Vladimirov GK, Philpott CC, Kagan VE. Achieving Life through Death: Redox Biology of Lipid Peroxidation in Ferroptosis. Cell Chem Biol. 2020 Apr 16;27(4):387-408. doi: 10.1016/j.chembiol.2020.03.014. Epub 2020 Apr 9. 


Anthony Cyr, Yanjun Zhong, Steven E. Reis, Rami A. Namas, Amoscato AA, Brian Zuckerbraun, Jason Sperry, Ruben Zamora, Yoram Vodovotz, Timothy R. Billiar. Analysis of the plasma metabolome after trauma, novel circulating sphingolipid signatures, and in-hospital outcomes. J. Am College Surgeons. 2021 Mar 232:276-287.


L.J. Sparvero, H. Tian, Amoscato AA, W.-Y. Sun, T.S. Anthonymuthu, Y.Y. Tyurina, O. Kapralov, S. Javadov, R.-R. He, S.C. Watkins, N. Winograd, V.E. Kagan, H. Bayir. Direct mapping of phospholipid ferroptotic death signals and cells and tissues by gas cluster ion beam secondar ion mass spectrometry (GCIB-SIMS). Angew. Chem. Int. Ed. 2021, 60:11784.

H. Tian, L.J. Sparvero, T.S. Anthonymuthu, W.-Y. Sun, A.A. Amoscato, R.-R. He, H. Bayir, V.E Kagan, N. Winograd. Successive high-resolution (H2O)n-GCIB and C60-SIMS imaging integrates multip-omics in different cell types in breast cancer tissue. Anal. Chem. (in press).

Andrew  Amoscato